| 货号 | 66012S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB,IP |
| 目标/特异性 | PLEKHM1 Antibody recognizes endogenous levels of total PLEKHM1 protein. |
| 使用方法 | Western Blotting (1:1000) Immunoprecipitation (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Pleckstrin homology domain-containing protein family member 1 (PLEKHM1) is a multivalent endolysosomal adaptor protein that contains an N-terminal RUN domain, two internal PH domains, and a C-terminal C1 domain/zinc-finger-like motif. Research studies have shown that PLEKHM1 plays a role in bone resorption by regulating the acidification of endosomal vesicles of osteoclasts. Loss-of-function mutations in PLEKHM1promote osteoclast failure and impair bone resorption, which leads to osteopetrosis (1-3). Recent studies have shown that PLEKHM1 serves as a nexus for different trafficking pathways of the endolysosomal network by serving as the platform for the recruitment of Rab7, HOPS complex, and LC3/GABARAP family members. Indeed, loss of PLEKHM1 impairs the lysosomal degradation of cell-surface receptors and autophagic clearance of protein aggregates (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 140 |
| 参考文献 | 1 . Van Wesenbeeck, L. et al. (2007) J Clin Invest 117, 919-30. 2 . Del Fattore, A. et al. (2008) J Bone Miner Res 23, 380-91. 3 . Fujiwara, T. et al. (2016) JCI Insight 1, e86330. 4 . McEwan, D.G. et al. (2015) Mol Cell 57, 39-54. 5 . McEwan, D.G. et al. (2015) Cell Host Microbe 17, 58-71. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PLEKHM1 protein (hPLEKHM1-Myc/DDK; +), using PLEKHM1 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Western blot analysis of extracts from various cell lines using PLEKHM1 Antibody. | |
Immunoprecipitation of PLEKHM1 from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Normal Rabbit IgG #2729, and lane 3 is PLEKHM1 Antibody. Western blot analysis was performed using PLEKHM1 Antibody. |
