| 货号 | 40804S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | TNFRSF8/CD30 Antibody recognizes endogenous levels of total TNFRSF8/CD30 protein. |
| 使用方法 | Western Blotting (1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | TNFRSF8/CD30 is a type-I transmembrane glycoprotein that is a member of the TNFR superfamily. CD30 is synthesized as a precursor protein that undergoes extensive posttranslational modification before becoming embedded in the plasma membrane as a 120-kDa transmembrane protein (1,2). The expression of CD30 is upregulated in activated T-cells and may trigger costimulatory signaling pathways upon its engagement (3,4). While its expression is normally restricted to subsets of activated T-cells and B-cells, CD30 expression is robustly upregulated in hematologic malignancies, such as Hodgkin’s lymphoma (HL), anaplastic large cell lymphoma (ALCL), and adult T-cell leukemia, thus making it an attractive target for therapeutic intervention (5,6). Research studies have suggested that in certain disease contexts, CD30 recruits TRAF2 and TRAF5 adaptor proteins to drive NF-kappa B activation, aberrant cell growth, and cytokine production (7-9). CD30 signaling is also regulated by TACE-dependent proteolytic cleavage of its ectodomain, which results in reduced CD30L-dependent activation of CD30+ cells (10, 11). |
| 存放说明 | -20C |
| 计算分子量 | 90 (precursor), 120 (mature) |
| 参考文献 | 1 . Froese, P. et al. (1987) J Immunol 139, 2081-7. 2 . Nawrocki, J.F. et al. (1988) J Immunol 141, 672-80. 3 . Del Prete, G. et al. (1995) J Exp Med 182, 1655-61. 4 . Gilfillan, M.C. et al. (1998) J Immunol 160, 2180-7. 5 . Stein, H. et al. (1985) Blood 66, 848-58. 6 . Chiarle, R. et al. (1999) Clin Immunol 90, 157-64. 7 . Horie, R. et al. (2002) Am J Pathol 160, 1647-54. 8 . Horie, R. et al. (2002) Oncogene 21, 2493-503. 9 . Horie, R. et al. (2004) Cancer Cell 5, 353-64. 10 . Hansen, H.P. et al. (2000) J Immunol 165, 6703-9. 11 . Gruss, H.J. et al. (1997) Immunol Today 18, 156-63. |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TNFRSF8/CD30 protein (hCD30-Myc/DDK; +), using TNFRSF8/CD30 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Western blot analysis of extracts from various cell lines using TNFRSF8/CD30 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, TNFRSF8/CD30 protein is not detected in either HPB-ALL or U-937 cells. KARPAS-299 cell line source: Dr. Abraham Karpas at the University of Cambridge. | |
Western blot analysis of extracts from KARPAS-299 cells, untreated (-) or treated with TPA #4174 (200nM, 90min; +), using TNFRSF8/CD30 Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). KARPAS-299 cell line source: Dr. Abraham Karpas at the University of Cambridge. |
