| 货号 | 40180S |
| 反应种属 | Human/Moneky |
| 来源宿主 | Rabbit |
| 应用 | W IP |
| 目标/特异性 | PJA2 Antibody recognizes endogenous levels of total PJA2 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Praja-2 (PJA2/RNF131) is a RING-H2-type E3 ubiquitin ligase. PJA2 is highly expressed in neural tissue and research studies have shown that PJA2 plays a role modulating synaptic archictecture and long-term memory by promoting the ubiquitin-dependent proteolysis of NOGO-A and PKA regulatory subunits (1,2). Research studies have also shown that PJA2 sustains gliobastoma growth by driving the ubiquitin-dependent proteasomal degradation of MOB, which inactivates the LATS tumor suppressor kinase of the Hippo pathway (3). |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Sepe, M. et al. (2014) Proc Natl Acad Sci U S A 111, 15729-34. 2 . Lignitto, L. et al. (2011) Nat Cell Biol 13, 412-22. 3 . Lignitto, L. et al. (2013) Nat Commun 4, 1822. |
Immunoprecipitation of PJA2 from 293T cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Normal Rabbit IgG #2729, and lane 3 is immunoprecipitation with PJA2 Antibody. Western blot analysis was performed with PJA2 Antibody. Anti-Rabbit HRP-conjugated light chain-specific secondary antibody was used for detection. | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PJA2 protein (hPJA2-Myc/DDK; +), using PJA2 Antibody. | |
Western blot analysis of extracts from various cell lines using PJA2 Antibody. |
