| 货号 | 5886S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | γ-Tubulin Antibody recognizes endogenous levels of total γ-tubulin protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. As a critical part of the microtubule-organizing center (MTOC), the third member of the tubulin superfamily, γ-tubulin, is required for microtubule nucleation as well as centrosome duplication and spindle assembly (1,2, reviewed in 3). γ-tubulin forms complexes of two different sizes: γ-tubulin small complex (γ-TuSC) and the larger γ-tubulin ring complex (γ-TuRC). Each complex consists of a number of γ-tubulin complex proteins (GCPs) with γ-tubulin itself being considered GCP1. GCP2-6 all share sequence similarity in 5 different regions and it is thought that these areas could play a role in the proper folding of the proteins (4). γ-TuSC is composed of two γ-tubulin molecules as well as GCP2 and GCP3. γ-TuRC is made up of a ring of multiple copies of γ-TuSC in addition to GCP4, 5, and 6. Another protein, GCP-WD/NEDD1, which lacks sequence similarity with the other GCPs, associates with the γ-TuRC. GCP-WD/NEDD1 has been shown to regulate localization of the γ-TuSC to spindles and centrosomes (5-8). In mammals, phosphorylation of γ-tubulin at Ser131 by SADB controls the activity of the γ-TuRC. The hypothesis is that this phosphorylation stabilizes the protein in a conformation that stimulates centrosome amplification (9). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
| 参考文献 | 1 . Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47. 2 . Loncarek, J. and Khodjakov, A. (2009) Mol Cells 27, 135-42. 3 . Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53. 4 . Murphy, S.M. et al. (2001) Mol Biol Cell 12, 3340-52. 5 . Raynaud-Messina, B. and Merdes, A. (2007) Curr Opin Cell Biol 19, 24-30. 6 . Schiebel, E. (2000) Curr Opin Cell Biol 12, 113-8. 7 . Lüders, J. et al. (2006) Nat Cell Biol 8, 137-47. 8 . Haren, L. et al. (2009) PLoS One 4, e5976. 9 . Alvarado-Kristensson, M. et al. (2009) Nat Cell Biol 11, 1081-92. |
Western blot analysis of extracts from various cell lines using γ-Tubulin Antibody.Western blot analysis of extracts from various cell lines using γ-Tubulin Antibody. 使用γ-Tubulin Antibody，免疫印迹（Western Blot）分析不同细胞中γ-Tubulin。 |
