| 货号 | 4967S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/D.melanogaster/Zebrafish/Bovine |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | β-Actin Antibody detects endogenous levels of β-actin. This antibody may cross-react with the γ-actin (cytoplasmic) isoform. It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). |
| 存放说明 | -20C |
| 计算分子量 | 45 |
| 参考文献 | 1 . Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55. 2 . Condeelis, J. (2001) Trends Cell Biol 11, 288-93. 3 . Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987. 4 . Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238. 5 . Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256. 6 . Du, J. et al. (2004) J. Clin. Invest. 113, 115-123. |
Western blot analysis of extracts from HeLa, C2C12, C6, COS, MvLu cells and guinea pig neutrophils (GPN), using β-Actin Antibody.使用β-Actin Antibody，免疫印迹（Western Blot）分析HeLa、C2C12、C6、COS、MvLu和guinea pig neutrophils (GPN)细胞系β-Actin蛋白水平。 |
