| 货号 | 5114S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | SQSTM1/p62 Antibody detects endogenous levels of total SQSTM1/p62 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy (1-4). It was first identified as a protein that binds to the SH2 domain of p56Lck (5), and independently found to interact with PKCζ (6,7). It was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome (8). Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway (9). Protein aggregates formed by SQSTM1 can be degraded by the autophagosome (4,10,11). SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome (12). Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress (3). Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity. |
| 存放说明 | -20C |
| 计算分子量 | 62 |
| 参考文献 | 1 . Kirkin, V. et al. (2009) Mol Cell 34, 259-69. 2 . Seibenhener, M.L. et al. (2007) FEBS Lett 581, 175-9. 3 . Komatsu, M. et al. (2010) Nat Cell Biol 12, 213-23. 4 . Bjørkøy, G. et al. (2006) Autophagy 2, 138-9. 5 . Joung, I. et al. (1996) Proc Natl Acad Sci USA 93, 5991-5. 6 . Sanchez, P. et al. (1998) Mol Cell Biol 18, 3069-80. 7 . Puls, A. et al. (1997) Proc Natl Acad Sci USA 94, 6191-6. 8 . Vadlamudi, R.K. et al. (1996) J Biol Chem 271, 20235-7. 9 . Wooten, M.W. et al. (2005) J Biol Chem 280, 35625-9. 10 . Bjørkøy, G. et al. (2005) J Cell Biol 171, 603-14. 11 . Komatsu, M. et al. (2007) Cell 131, 1149-63. 12 . Pankiv, S. et al. (2007) J Biol Chem 282, 24131-45. |
Western blot analysis of extracts from various cell lines using SQSTM1/p62 Antibody. Western blot分析多种细胞系的细胞提取物，使用抗体是SQSTM1/p62 Antibody。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with mouse SQSTM1 isoform c (mSQSTM1c) construct (+), using SQSTM1/p62 Antibody. Western blot 分析293T细胞的细胞提取物，对照转染(-)或转染mouse SQSTM1 isoform c (mSQSTM1c) 质粒(+)，使用抗体是SQSTM1/p62 Antibody。 |
