货号 | 2272S |
反应种属 | All |
来源宿主 | Rabbit |
应用 | W/IF-IC/F |
使用方法 | WB(1:1000) F (1:800) IF-IC (1:400) |
供应商 | CST |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | -20C |
Western blot analysis of cell extracts alone (lane 1), cells expressing Myc tagged to the carboxy terminus of the protein (lane 2) and Myc tagged to the amino terminus of the protein (lane 3), using Myc-Tag Antibody.Western blot分析细胞提取物(泳道1),羧基末端带有Myc标签的蛋白(泳道2 )和氨基末端带有Myc标签的蛋白(泳道3),所用抗体为Myc-Tag Antibody。 | |
Flow cytometric analysis of COS cells, untreated (blue) or Myc transfected (green), using Myc-Tag Antibody.流式细胞术分选COS细胞,蓝色为未处理组,绿色为Myc转染的细胞,所用抗体为Myc-Tag Antibody。 | |
Confocal immunofluorescent analysis of COS cells transfected with a Myc-tagged protein (left) or untransfected (right) using Myc-Tag Antibody. Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦免疫荧光分析COS细胞Myc标签蛋白转染的细胞(左图)和未转染组(右图),肌动蛋白丝采用DY-554 phalloidin (红色)标记。 |