| 货号 | 2972S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | mTOR Antibody detects endogenous levels of total mTOR. It cross-reacts weakly with some other proteins based on Western analysis. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 计算分子量 | 289 |
Western blot analysis of extracts from 293 cells (starved for 16 hours), untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) antibody #2971 (upper) or mTOR Antibody (lower). Western blot方法检测饥饿处理16小时的293细胞提取物：未处理和EGF (100 ng/ml)处理30分钟，使用的抗体为Phospho-mTOR (Ser2448) antibody #2971(上图) 和 mTOR Antibody (下图)。 | |
Western blot analysis of extracts from HeLa cells, transfected with either control siRNA (-) or mTOR siRNA (+). mTOR was detected using mTOR Antibody #2972, and eIF4B was detected using eIF4B Antibody #3592. The mTOR Antibody confirms silencing of mTOR expression, and the eIF4B Antibody is used to control for loading and siRNA specificity. Western blot方法检测Hela细胞提取物：用对照siRNA（-）和mTOR siRNA（+） 转染； 使用mTOR Antibody #2972检测mTOR，eIF4B Antibody #3592检测eIF4B；mTOR抗体确定mTOR表达的沉默， eIF4B 抗体作为上样量和siRNA特异性的对照。 |
