货号 | 2448S |
反应种属 | All |
来源宿主 | Rabbit |
应用 | W/IP/IF-IC |
使用方法 | WB(1:1000) IP (1:50) IF-IC (1:500) |
供应商 | CST |
背景 | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged proteins biochemical properties. |
存放说明 | -20C |
计算分子量 | N/A |
Confocal immunofluorescent analysis of parental COS cells (left), COS cells expressing an N-terminal Glu-Glu-tagged protein (middle), and COS cells expressing a C-terminal Glu-Glu-tagged protein (right), labeled with Glu-Glu Tag Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光公共聚焦免疫荧光分析母COS细胞(左图),中图为表达N末端带有Glu-Glu标签的蛋白COS细胞,右图为表达C末端带有Glu-Glu标签的蛋白COS细胞,所用抗体为Glu-Glu Tag Antibody(绿色)。肌动蛋白丝采用 Alexa Fluor ® 555 phalloidin (红色),蓝色伪彩为荧光DNA燃料,产品信息为DRAQ5 ® #4084。 | |
Western blot analysis of total cell lysates from Cos cells (lane 1), Cos cells expressing an N-terminal Glu-Glu-tagged protein (lane 2), and Cos cells expressing a C-terminal Glu-Glu-tagged protein (lane 3), using Glu-Glu Tag Antibody.Weatern blot分析cos细胞总提取物(泳道1),泳道2 为表达N末端带有Glu-Glu标签的蛋白COS细胞,泳道3 为表达C末端带有Glu-Glu标签的蛋白COS细胞,所用抗体为Glu-Glu Tag Antibody。 |