| 货号 | 9662S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa). |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1000) |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | -20C |
| 计算分子量 | 17, 19, 35 |
Immunohistochemical staining of paraffin-embedded human tonsil, showing cytoplasmic localization, using Caspase-3 Antibody. 免疫组化分析石蜡包埋的人扁桃体切片，显示caspase-3定位胞浆，抗体采用Caspase-3 Antibody。 | |
Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), and NIH/3T3 cells, untreated or staurosporine-treated (1uM, 3hrs), using Caspase-3 Antibody. Western blot分析未处理或etoposide (25uM, 5hrs)处理的Jurkat 细胞，以及未处理或 staurosporine (1uM, 3hrs)处理的NIH/3T3 细胞，抗体采用Caspase-3 Antibody. |
