| 货号 | 2772S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membranes permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4). |
| 存放说明 | -20C |
| 计算分子量 | 20 |
Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody. Western blot 免疫印迹。用Bax Antibody研究 293 (人源)、COS (猴子来源)、L929 (小鼠源) 和 PC12 (大鼠) 细胞的细胞提取液。 | |
Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate. 用Bax Antibody经免疫沉淀分析Jurkat细胞提取液中的Bax，泳道1 是裂解液的对照；泳道2是只有抗体；泳道3是抗体加上裂解液。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bax siRNA I (+) or SignalSilence® Bax siRNA II (+), using Bax Antibody and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA. Western blot免疫印迹分析转染了100 nM SignalSilence® Control siRNA (荧光偶联) #6201 (-),SignalSilence® Bax siRNA I (+) 或SignalSilence® Bax siRNA II(+)的HeLa细胞的细胞提取液，所用抗体为Bax Antibody 和α-Tubulin (11H10) Rabbit mAb 兔单抗#2125。Bax Antibody证实对Bax表达的沉默,α-Tubulin (11H10) Rabbit mAb 兔单抗作为上样量的对照，并验证Bax siRNA的特异性。 |
