| 货号 | 2532S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | AMPKalpha Antibody detects endogenous levels of AMPKα protein. The antibody detects both the α1 and α2 isoforms of the catalytic subunit, but it does not detect the regulatory β or γ subunits. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis (1). AMPK is a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, each of which is encoded by two or three distinct genes (α1, 2; β1, 2; γ1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia, and ischemia (1). The tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPKα at Thr172 in the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKα is also phosphorylated at Thr258 and Ser485 (for α1; Ser491 for α2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The β1 subunit is post-translationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the β1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKγ subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1). |
| 存放说明 | -20C |
| 计算分子量 | 62 |
Western blot analysis of extracts from HEK293 cells, untreated or oligomycin-treated (0.5μM) for the indicated times, using AMPKα Antibody. 对HEK293细胞抽提液，未处理或0.5uM寡霉素处理适当时间，使用AMPKα Antibody进行Western blot分析。 | |
Immunprecipitation of AMPK from untreated 3T3 cell extracts using AMPK antibody (Lane 1). Lane 2: No antibody control. Lane 3: Input control. 对3T3细胞裂解液使用AMPK antibody（列1）进行AMPK免疫共沉淀分析。列2：无抗体对照。列3：输入对照。 | |
Western blot analysis of extracts from various cell lines using AMPK alpha Antibody. 对多个细胞系使用AMPK alpha Antibody进行Western blot分析。 |
