| 货号 | 18070T |
| 目标/特异性 | Each antibody in the Mutant Ras Antibody Sampler kit detects endogenous levels of its target protein. Ras (D2C1) Rabbit mAb recognizes endogenous levels of total Ras protein, specifically K-Ras and N-Ras. This antibody does not cross-react with H-Ras or R-Ras. |
| 供应商 | CST |
| 背景 | The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6). Additional oncogenic mutations of Ras have been observed at varying frequencies at codons 12, 13, and 61. The Gly12 to Val12 (G12V) mutation has been detected in a number of different cancers, including colorectal and thyroid cancer (7,8). |
| 存放说明 | -20C |
| 参考文献 | 1 . Boguski, M.S. and McCormick, F. (1993) Nature 366, 643-54. 2 . Avruch, J. et al. (1994) Trends Biochem Sci 19, 279-83. 3 . Buday, L. and Downward, J. (1993) Cell 73, 611-20. 4 . Huang, D.C. et al. (1993) Mol Cell Biol 13, 2420-31. 5 . Bos, J.L. (1989) Cancer Res 49, 4682-9. 6 . Ma, J. and Karplus, M. (1997) J Mol Biol 274, 114-31. 7 . Prior, I.A. et al. (2012) Cancer Res 72, 2457-67. 8 . Winder, T. et al. (2009) Oncol Rep 21, 1283-7. |
