| 货号 | 9761T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Cleaved Caspase-6 (Asp162) Antibody detects endogenous levels of the large subunit of caspase-6 resulting from cleavage at aspartic acid162. This antibody does not recognize other cleaved caspases. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2). Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3). The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,5). One of the major targets for caspase-6 is the membrane associated protein lamin A (6). The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death. |
| 存放说明 | -20C |
| 计算分子量 | 18 |
| 参考文献 | 1 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 2 . Faleiro, L. et al. (1997) EMBO J 16, 2271-81. 3 . Li, P. et al. (1997) Cell 91, 479-489. 4 . Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292. 5 . MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479. 6 . Orth, K. et al. (1996) J Biol Chem 271, 16443-6. |
Western blot analysis of Jurkat cell extracts, untreated or etoposide-treated, and NIH/3T3 and C6 cell extracts, untreated or staurosporine-treated, using Cleaved Caspase-6 (Asp162) Antibody. 对未处理或 etoposide 处理的Jurkat细胞，未处理或 staurosporine 处理的NIH/3T3 和 C6 细胞进行western Blot分析，抗体采用Cleaved Caspase-6 (Asp162) Antibody。 |
