| 货号 | 2844T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-C/EBPalpha (Thr222/226) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at threonine 222 and 226. This antibody does not cross-react with other phosphorylated C/EBP isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4). |
| 存放说明 | -20C |
| 计算分子量 | 30, 42, 45 |
| 参考文献 | 1 . Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548. 2 . Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610. 3 . Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919. 4 . Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441. |
Western blot analysis of extracts from U937 cells treated with either LiCl or NaCl for the indicated times, using Phopho-C/EBPalpha (Thr222/226) Antibody (upper) and C/EBPalpha antibody (lower). C/EBPalpha phosphorylation at Thr222/226 is abolished by the specific GSK3 inhibitor LiCl, but not by NaCl, indicating that phosphorylation at these sites are depends on GSK3 kinase. 对U937细胞抽提液，LiCl或NaCl处理适当时间，使用Phopho-C/EBPalpha (Thr222/226) Antibody（上图）或C/EBPalpha antibody（下图）进行Western blot分析。C/EBPalpha在Thr222/226的磷酸化被特异性的GSK3抑制剂LiCl消除，但不能被氯化钠消除，这表明这些位点的磷酸化需要GSK3激酶。 |
