| 货号 | 2875T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Bcl-2 (Thr56) Antibody (Human Specific) detects endogenous levels of Bcl-2 only when phosphorylated at threonine 56. It does not cross-react with non-phosphorylated Bcl-2 or with other Bcl-2 family members at endogenous levels. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7). |
| 存放说明 | -20C |
| 计算分子量 | 28 |
| 参考文献 | 1 . Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11. 2 . Zhu, L. et al. (1999) J Biol Chem 274, 33267-73. 3 . Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42. 4 . Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78. 5 . Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91. 6 . Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32. 7 . Deng, X. et al. (2001) J Biol Chem 276, 23681-8. |
Western blot analysis of Jurkat cells, untreated or treated with pacilitaxel for the indicated times, using Phospho-Bcl-2 (Thr56) Antibody (Human Specific) (top) or Bcl-2 Antibody (Human Specific) #2872 (bottom). Western blot免疫印迹分析未经处理的和经pacilitaxel 处理一定时间的 Jurkat 细胞的细胞提取液，所用抗体是Phospho-Bcl-2 (Thr56) Antibody (Human Specific) (上图) 或者 Bcl-2 Antibody (Human Specific) #2872 (下图) 。 |
