| 货号 | 9111T |
| 反应种属 | Human,Mouse,Rat,Monkey,D. melanogaster,Xenopus,S. cerevisiae, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-cdc2 (Tyr15) Antibody detects endogenous levels of cdc2, CDK2 and CDK5 only when phosphorylated at tyrosine 15. Based on sequence similarity, the antibody may also cross-react with CDK3. The antibody does not cross-react with CDK4, CDK6 or CDK7. It does detect the yeast orthologue of cdc2 (cdc28) when phosphorylated at tyrosine 19. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5).cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。 |
| 存放说明 | -20C |
| 计算分子量 | 34 |
| 参考文献 | 1 . Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001. 2 . Norbury, C. et al. (1991) EMBO J 10, 3321-9. 3 . McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85. 4 . Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71. 5 . Hunter, T. (1995) Cell 80, 225-36. |
Western blot analysis of extracts from Saos cells, either untreated or treated with hydroxyurea or nocodazole, using Phospho-cdc2 (Tyr15) Antibody #9111 (upper) or cdc2 Antibody #9112 (lower). western blot方法检测未处理和羟基脲或 nocodazole处理的Saos细胞提取物，使用的抗体为Phospho-cdc2 (Tyr15) Antibody #9111 (上图) 或cdc2 Antibody #9112 (下图)。 |
