| 货号 | 2974T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-mTOR (Ser2481) Antibody detects endogenous levels of mTOR only when phosphorylated at serine 2481. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9). |
| 存放说明 | -20C |
| 计算分子量 | 289 |
| 参考文献 | 1 . Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22. 2 . Brown, E.J. et al. (1994) Nature 369, 756-8. 3 . Sabatini, D.M. et al. (1994) Cell 78, 35-43. 4 . Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. 5 . Dennis, P.B. et al. (2001) Science 294, 1102-5. 6 . Fang, Y. et al. (2001) Science 294, 1942-5. 7 . Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31. 8 . Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23. 9 . Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7. |
Western blot analysis of extracts from 293 cells (starved for 30 hours), untreated or treated with 20% FBS for 30 minutes, using Phospho-mTOR (Ser2481) Antibody (upper) or control mTOR Antibody #2972 (lower). Western blot方法检测饥饿处理30小时的293细胞提取物：未处理和20% FBS处理30分钟，使用的抗体为 Phospho-mTOR (Ser2481) Antibody(上图) 和 control mTOR Antibody #2972 (下图)。 |
