| 货号 | 9545T |
| 反应种属 | Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Cleaved PARP (Asp214) Antibody (Rat Specific) detects endogenous levels of the large fragment of rat PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). |
| 存放说明 | -20C |
| 计算分子量 | 89 |
| 参考文献 | 1 . Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358. 2 . Lazebnik, Y. A. et al. (1994) Nature 371, 346-347. 3 . Cohen, G.M. (1997) Biochem. J. 326, 1-16. 4 . Nicholson, D. W. et al. (1995) Nature 376, 37-43. 5 . Tewari, M. et al. (1995) Cell 81, 801-809. 6 . Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539. |
Western blot analysis of C6 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Rat Specific). |
