| 货号 | 3001T |
| 反应种属 | Human/Mouse/Mink |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-p95/NBS1 (Ser343) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7).Nijmegen 断裂综合征(NBS)的主要特征有细胞周期检验点缺陷，生长迟缓，癌症倾向和离子辐射敏感性增强(1)。辐射修复DNA双链断裂依赖于多功能复合体，包括Rad50, Mre11和NBS1基因产物p95/NBS1 (也称作 p95 或nibrin) (2)。p95/NBS1是一种在细胞周期调控和DNA修复中发现的一种蛋白质，具有叉头相关结构域和重复序列的羧基末端(1,3)。共济失调毛细血管扩张症和NBS临床上以及细胞表型之间的重叠表明ATM和p95/NBS1在同一个生化通路中起作用。ATM和p95/NBS1相互作用并能够在暴露电离辐射之后磷酸化p95/NBS1蛋白丝氨酸343位(4-7)。 |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Chrzanowska, K.H. et al. (2012) Orphanet J Rare Dis 7, 13. 2 . Lee, J.H. et al. (2013) J Biol Chem 288, 12840-51. 3 . Hari, F.J. et al. (2010) EMBO Rep 11, 387-92. 4 . Wang, H. et al. (2013) PLoS Genet 9, e1003277. 5 . Zhao, S. et al. (2000) Nature 405, 473-7. 6 . Wen, J. et al. (2013) Oncogene 32, 4448-56. |
Western blot analysis of extracts from MvILu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody.western blot方法检测不同时间点，由紫外和羟基脲（HU）分别处理的MvILu细胞提取物，使用的抗体为Phospho-p95/NBS1 (Ser343) Antibody。 |
