| 货号 | 3021T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody detects endogenous levels of Tyr1131-phosphorylated IGF-I receptor and Tyr1146-phosphorylated insulin receptor. The antibody cross-reacts with activated PDGF, FGF and EGF receptors, ErbB2 and c-Met. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93. 2 . Baserga, R. (2000) Oncogene 19, 5574-81. 3 . Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8. 4 . Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81. 5 . Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60. 6 . Baserga, R. (1999) Exp Cell Res 253, 1-6. 7 . White, M.F. et al. (1985) J Biol Chem 260, 9470-8. 8 . White, M.F. et al. (1988) J Biol Chem 263, 2969-80. |
Western blot analysis of extracts from 293 cells, untreated or IGF-I-treated (100 nM for 2 minutes), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IGF-I Receptor antibody (lower). 对293细胞抽提液，未处理或100nM IGF-I处理2分钟，使Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody（上图）或对照IGF-I受体抗体（下图）进行Western blot分析。 | |
Western blot analysis of extracts from 3T3-L1 adipocytes, untreated or insulin-treated (100 nM for the indicated times), using Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody (upper) or control IR antibody (lower). 对3T3-L1脂肪细胞抽提液，未处理或100nM胰岛素处理适当时间，使Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody（上图）或对照IR抗体（下图）进行Western blot分析。 |
