| 货号 | 3084T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-C/EBPbeta (Thr235) Antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37. This antibody does not cross-react with other phosphorylated C/EBPs. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6). |
| 存放说明 | -20C |
| 计算分子量 | 19 LIP. 36 LAP. 38 LAP. |
| 参考文献 | 1 . Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548. 2 . Calkhoven, C.F. et al. (2000) Genes Dev 14, 1920-32. 3 . Wegner, M. et al. (1992) Science 256, 370-3. 4 . Trautwein, C. et al. (1993) Nature 364, 544-7. 5 . Nakajima, T. et al. (1993) Proc Natl Acad Sci U S A 90, 2207-11. 6 . Buck, M. et al. (1999) Mol Cell 4, 1087-92. |
Western blot analysis of extracts from adipocytes (differentiated 3T3-L1) treated with insulin for the indicated times, using Phospho-C/EBPbeta (Thr235) Antibody.对脂肪细胞（分化的3T3-L1）抽提液，胰岛素处理适当时间，使用Phospho-C/EBPbeta (Thr235)抗体进行Western blot分析。 |
