| 货号 | 3114T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-VASP (Ser239) Antibody detects endogenous levels of VASP only when phosphorylated at serine 239. The antibody does not cross-react with phosphorylated VASP homologues, such as Mena. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 48, 50 |
| 参考文献 | 1 . Butt, E. et al. (1994) J Biol Chem 269, 14509-17. 2 . Ball, L.J. et al. (2000) EMBO J 19, 4903-14. 3 . Machesky, L.M. (2000) Cell 101, 685-8. 4 . Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35. 5 . Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25. 6 . Oelze, M. et al. (2000) Circ Res 87, 999-1005. 7 . Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6. |
Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser239) Antibody. 使用Phospho-VASP (Ser239) Antibody，免疫印迹(Western blot)分析NIH/3T3和C6细胞，细胞分别经untreated、8-Br-cGMP-treated、8-Br-cAMP-treated或forskolin-treated如图所示。 | |
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Forskolin #3828 (+), using Phospho-VASP (Ser239) Antibody (upper) and VASP (9A2) Rabbit mAb #3132 (lower). |
