| 货号 | 9429T |
| 反应种属 | Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific) detects endogenous levels of mouse and rat caspase-8 when cleaved at Asp387. This antibody will detect cleavage products containing the pro-domain with the p18 subunit (p43) as well as the p18 subunit alone. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. |
| 存放说明 | -20C |
| 计算分子量 | 18, 43 |
| 参考文献 | 1 . Muzio, M. et al. (1996) Cell 85, 817-27. 2 . Boldin, M.P. et al. (1996) Cell 85, 803-15. 3 . Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9. |
Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by TNF-α #8902 (20 ng/ml, 4 hours), using Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific). | |
Western blot analysis of extracts from COS cells, mock transfected (-) or transfected with a construct overexpressing mouse caspase-8 (+), using Cleaved Caspase-8 (Asp387) Antibody (Mouse Specific). |
