| 货号 | 3234T |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Gab1 (Tyr307) Antibody detects endogenous levels of Gab1 only when phosphorylated at tyrosine 307. The antibody cross-reacts with phosphorylated Gab2 and may also cross-react with phosphorylated Gab3. The antibody may cross-react with activated receptor tyrosine kinases (RTKs). |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9). |
| 存放说明 | -20C |
| 计算分子量 | 115 |
| 参考文献 | 1 . Holgado-Madruga, M. et al. (1996) Nature 379, 560-564. 2 . Weidner, K.M. et al. (1996) Nature 384, 173-176. 3 . Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117. 4 . Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65. 5 . Lehr, S. et al. (1999) Biochemistry 38, 151-159. 6 . Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923. 7 . Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8. 8 . Gual, P. et al. (2000) Oncogene 19, 1509-18. 9 . Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7. |
Western blot analysis of extracts from HEK293 cells, untreated or treated with In1B for indicated times, using Phospho-Gab1 (Tyr307) Antibody (upper) or Gab1 Antibody #3232 (lower). Western blot 检测分析HEK293细胞提取物，未处理的和用 In1B处理特定时间的；使用的抗体是 Phospho-Gab1 (Tyr307) Antibody（上图）和Gab1 Antibody #3232 （下图）。 |
