| 货号 | 3251T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Caveolin-1 (Tyr14) Antibody detects endogenous levels of caveolin-1 only when phosphorylated at tyrosine 14. The antibody does not cross-react with paxillin, caveolin-2, -3 or caveolin-1beta, the short isoform of caveolin-1. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 21-24 kDa integral proteins, caveolins, are the principal structural components of the cholesterol/sphingolipid-enriched plasma membrane microdomain caveolae. Three members of the caveolin family (caveolin-1, -2 and -3) have been identified with different tissue distributions. Caveolins form hetero- and homooligomers, which interact with cholesterol and other lipids (1). Caveolins are involved in diverse biological functions, including vesicular trafficking, cholesterol homeostasis, cell adhesion and apoptosis, and are also implicated in neurodegenerative disease (2). Caveolins also interact with multiple signaling molecules, such as Gα subunit, tyrosine kinase receptors, PKCs, Src family tyrosine kinases and eNOS (1,2). It is believed that caveolins serve as scaffolding proteins for the integration of signal transduction. Phosphorylation at Tyr14 is essential for caveolin association with SH2 or PTB domain-containing adaptor proteins, such as GRB7 (3-5). Phosphorylation at Ser80 regulates caveolin binding to the ER membrane and entry into the secretory pathway (6).caveolins蛋白分子量是21-24 kDa，其富含胆固醇/鞘磷脂膜的质膜微区caveolae的主要结构成分。caveolin蛋白家族三个成员（caveolin-1、-2和-3）在不同组织分布已经被鉴定。Caveolins蛋白形成异源二聚体和同源二聚体，其能与胆固醇和其它脂质相互作用 (1)。Caveolins蛋白涉及不同生物功能，包括囊泡运输、胆固醇体内平衡、细胞粘附和凋亡，并且也涉及到神经退行性疾病 (2)。Caveolins蛋白也与多种信号分子相互作用，例如Gα亚型、酪氨酸激酶受体、PKCs、Src家族酪氨酸激酶和eNOS(1,2)。有研究认为caveolins 蛋白作为对信号转导的整合的支架蛋白 。在 Tyr14位点磷酸化对caveolin蛋白与SH2或PTB区域包含受体蛋白例如GRB7的联系有本质作用(3-5)。在Ser80 位点的磷酸化调节caveolin蛋白结合ER膜，并且进入分泌通路(6)。 |
| 存放说明 | -20C |
| 计算分子量 | 23, 25 |
| 参考文献 | 1 . Okamoto, T. et al. (1998) J Biol Chem 273, 5419-22. 2 . Smart, E.J. et al. (1999) Mol Cell Biol 19, 7289-304. 3 . Nomura, R. et al. (1999) Mol. Biol. Cell 10, 975-986. 4 . Volonte, D. et al. (2001) J. Biol. Chem. 276, 8094-8103. 5 . Lee, H. et al. (2000) Mol Endocrinol 14, 1750-75. 6 . Schlegel, A. et al. (2001) J Biol Chem 276, 4398-408. |
Western blot analysis of extracts from HeLa cells, untreated (-) or H2O2-treated (+), using Phospho-Caveolin-1 (Tyr14) Antibody (upper) and Caveolin-1 (D46G3) XP® Rabbit mAb #3267 (lower).使用Phospho-Caveolin-1 (Tyr14) Antibody (上图)和Caveolin-1 (D46G3) XP® Rabbit mAb #3267 (下图)，免疫印迹（Western Blot）分析HeLa细胞系Phospho-Caveolin-1 (Tyr14)和Caveolin-1蛋白水平，细胞分为未处理组(-)和H2O2处理组(+)。 |
