| 货号 | 3571T |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-CD19 (Tyr531) Antibody detects endogenous levels of CD19 only when phosphorylated at tyrosine 531. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
| 参考文献 | 1 . Tedder, T.F. et al. (1997) Immunity 6, 107-18. 2 . Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46. 3 . Fujimoto, M. et al. (2000) Immunity 13, 47-57. 4 . ORourke, L.M. et al. (1998) Immunity 8, 635-45. 5 . Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62. |
Western blot analysis of SDS extracts from untreated or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells using Phospho-CD19 (Tyr531) Antibody (upper) and control CD19 Antibody #3574 (lower).Western免疫印迹。用Phospho-CD19 (Tyr531) Antibody (上图)和 control CD19 Antibody #3574 (下图)研究未经处理的和经anti-human IgM treated (12 µg/ml,2 min) 处理的Ramos细胞的SDS细胞提取液。 |
