| 货号 | 3591T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-eIF4B (Ser422) Antibody detects eIF4B only when phosphorylated at Ser422. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on the MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translational activity of eIF4B (7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
| 参考文献 | 1 . Le, H. et al. (1997) J. Biol. Chem. 272, 16247-16255. 2 . Duncan, R.F. and Hershey, J.W. (1989) J. Cell Biol. 109, 1467-1481. 3 . Duncan, R.F. and Hershey, J.W. (1984) J. Biol. Chem. 259, 11882-11889. 4 . Duncan, R. and Hershey, J.W. (1985) J. Biol. Chem. 260, 5493-5497. 5 . Manzella, J.M. et al. (1991) J. Biol. Chem. 266, 2383-2389. 6 . Gingras, A.C. et al. (2001) Genes Dev 15, 807-26. 7 . van Gorp, A.G. et al. (2009) Oncogene 28, 95-106. 8 . Raught, B. et al. (2004) EMBO J 23, 1761-9. |
Western blot analysis of extracts from HeLa cells using Phospho-eIF4B (Ser422) Antibody (upper) or eIF4B Antibody #3592 (lower). 48 hours following siRNA transfection, cells were treated with Rapamycin (50 nM) and U0126 (10 µM) as indicated. 使用Phospho-eIF4B (Ser422) Antibody (上图)和eIF4B Antibody #3592 (下图)，免疫印迹（Western Blot）分析在HeLa细胞系中磷酸化eIF4B (Ser422)和eIF4B蛋白水平，细胞在siRNA转染后48小时，按图中所标分别使用Rapamycin (50 nM)和U0126 (10 µM)处理。 |
