| 货号 | 3584T |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho LAT (Tyr191) Antibody detects endogenous levels of LAT only when phosphorylated at tyrosine 191. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). |
| 存放说明 | -20C |
| 计算分子量 | 36, 38 |
| 参考文献 | 1 . Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-83. 2 . Zhang, W. et al. (1998) Cell 92, 83-92. 3 . Paz, P.E. et al. (2001) Biochem J 356, 461-71. 4 . Zhang, W. et al. (2000) J Biol Chem 275, 23355-61. |
Western blot analysis of SDS extracts from untreated or anti-CD3 treated (10 µg/ml for 2 minutes) human Jurkat cells after overnight serum starvation using Phospho-LAT (Tyr191) Antibody.Western免疫印迹。用Phospho-LAT (Tyr191) Antibody研究未经处理的或经anti-CD3 10 µg/ml 2 min处理的血清饥饿过夜的Jurkat细胞的SDS细胞提取液。 |
