| 货号 | 3601T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-BLNK (Tyr96) Antibody detects endogenous levels of BLNK only when phosphorylated at tyrosine 96. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | BLNK (B cell linker protein), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts on the interface between BCR-associated Syk and the downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino-terminus and a SH2 domain at its carboxy-terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules such as Btk, PLCgamma and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets such as HPK1 to recruit it to the BCR complex for signaling (5). |
| 存放说明 | -20C |
| 计算分子量 | 68, 70 |
| 参考文献 | 1 . Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5. 2 . Fu, C. et al. (1998) Immunity 9, 93-103. 3 . Ishiai, M. et al. (1999) Immunity 10, 117-25. 4 . Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-86. 5 . Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-39. |
Western blot analysis of SDS extracts from control or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells using Phospho-BLNK (Tyr96) Antibody. |
