Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody【科瑞奥博】

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Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody

货号： 3674T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	3674T
反应种属	Human/Mouse
来源宿主	Rabbit
应用	W
目标/特异性	Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody detects endogenous levels of myosin light chain 2 (smooth muscle) only when dually phosphorylated at threonine 18 and serine 19. The antibody does not cross-react with the cardiac isoform of myosin light chain 2.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	Myosin is composed of six polypeptide chains: two identical heavy chains and two pairs of light chains. Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC or RLC, LC20), has many isoforms depending on its distribution. In smooth muscle, MLC2 is phosphorylated at Thr18 and Ser19 by myosin light chain kinase (MLCK) in a Ca2+/calmodulin-dependent manner (1). This phosphorylation is correlated with myosin ATPase activity and smooth muscle contraction (2). ROCK also phosphorylates Ser19 of smooth muscle MLC2, which regulates the assembly of stress fibers (3). Phosphorylation of smooth muscle MLC2 at Ser1/Ser2 and Ser9 by PKC and cdc2 has been reported to inhibit myosin ATPase activity (4,5). Phosphorylation by cdc2 controls the timing of cytokinesis (5). Transgenic mice lacking phosphorylation sites on the cardiac muscle isoform show morphological and functional abnormalities (6).
存放说明	-20C
计算分子量	18
参考文献	1 . Ikebe, M. and Hartshorne, D.J. (1985) J Biol Chem 260, 10027-31. 2 . Tan, J.L. et al. (1992) Annu Rev Biochem 61, 721-59. 3 . Totsukawa, G. et al. (2000) J. Cell Biol. 150, 797-806. 4 . Ikebe, M. et al. (2000) J. Biol. Chem. 262, 9569-9573. 5 . Satterwhite, L. L. et al. (1992) J. Cell Biol. 118, 595-605. 6 . Sanbe, A. et al. (1999) J. Biol. Chem. 274, 21085-21094.

参考图片

Western blot analysis of extracts from HEK293 cells stimulated with ionophore A23187 for the indicated times, using Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody.

使用Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody，免疫印迹（Western Blot）分析HEK293细胞中Phospho-Myosin Light Chain 2 (Thr18/Ser19)蛋白水平，细胞如图时间所示给予ionophore A23187处理。