| 货号 | 3691T |
| 反应种属 | Human/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation. |
| 存放说明 | -20C |
| 计算分子量 | 105 |
| 参考文献 | 1 . Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889. 2 . Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943. 3 . Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792. 4 . Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34. 5 . Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529. 6 . Hovland, R. et al. (1999) FEBS Lett. 444, 97-101. 7 . Wang, X. et al. (2001) EMBO J. 20, 4370-4379. 8 . Knebel, A. et al. (2001) EMBO J. 20, 4360-4369. |
Western blot analysis of extracts from SW-13 cells (starved for 18 hours) treated with calf intestinal alkaline phosphatase (CIP) or 20% fetal bovine serum for 30 minutes, and extracts from HeLa cells (starved for 18 hours) treated with 200 nM TPA for 30 minutes, using Phospho-eEF2k (Ser366) Antibody (upper) or eEF2k Antibody #3692 (lower). 使用Phospho-eEF2k (Ser366) Antibody (上图)和eEF2k Antibody #3692 (下图)，免疫印迹（Western Blot）分析在SW-13细胞和HeLa细胞中磷酸化eEF2k和eEF2k蛋白水平， SW-13(饥饿处理18小时)细胞提取物分为牛小肠碱性磷酸酶(CIP)处理30分钟组和20%胎牛血清处理30分钟组。HeLa细胞(饥饿处理18小时)的提取物是经过200 nM TPA处理30分钟。 |
