| 货号 | 3751T |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-SHP-2 (Tyr542) Antibody detects endogenous levels of SHP-2 only when phosphorylated at Tyr542. This antibody may cross-react with activated receptor tyrosine kinases. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy-terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
| 参考文献 | 1 . Qu, C.K. (2000) Cell Res 10, 279-88. 2 . Maroun, C.R. et al. (2000) Mol Cell Biol 20, 8513-25. 3 . Zhang, S.Q. et al. (2002) Mol Cell Biol 22, 4062-72. 4 . Bennett, A.M. et al. (1994) Proc Natl Acad Sci USA 91, 7335-9. 5 . Lu, W. et al. (2001) Mol Cell 8, 759-69. 6 . Edouard, T. et al. (2007) Cell Mol Life Sci 64, 1585-90. |
Western blot analysis of extracts from C6 and NIH/3T3 cells, starved for 18 hours and either untreated or PDGF-treated (50ng/ml, 20 minutes), using Phospho-SHP-2 (Tyr542) Antibody (upper) or control SHP-2 Antibody #3752 (lower).Western blot方法检测细胞提取物：未经处理和PDGF(50ng/ml, 20 min)处理的18h无血清培养C6、NIH/3T3细胞。使用的抗体是Phospho-SHP-2 (Tyr542) Antibody (上图)和 control SHP-2 Antibody #3752(下图)。 |
