| 货号 | 3841T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody detects transfected levels of LIMK1 and LIMK2 only when phosphorylated at threonine 508 or 505. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Transfected Only |
| 背景 | LIM kinases (LIMK1 and LIMK2) are serine/threonine kinases that have two zinc finger motifs, known as LIM motifs, in their amino-terminal regulatory domains (1). LIM kinases are involved in actin cytoskeletal regulation downstream of Rho-family GTPases, PAKs and ROCK (2,3). PAK1 and ROCK phosphorylate LIMK1 or LIMK2 at the conserved Thr508 or Thr505 residues in the activation loop, increasing the LIMK activity (3-5). Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the amino-terminal Ser3 residue of cofilin (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
| 参考文献 | 1 . Okano, I. et al. (1995) J Biol Chem 270, 31321-30. 2 . Maekawa, M. et al. (1999) Science 285, 895-8. 3 . Edwards, D.C. et al. (1999) Nat Cell Biol 1, 253-9. 4 . Ohashi, K. et al. (2000) J Biol Chem 275, 3577-82. 5 . Sumi, T. et al. (2001) J Biol Chem 276, 670-6. 6 . Arber, S. et al. (1998) Nature 393, 805-809. 7 . Yang, N. et al. (1998) Nature 393, 809-812. |
Western blot analysis of extracts from COS cells, untransfected (lane 1), transfected with Wild-type LIMK1 (lanes 2 and 3) or with LIMK1 T508A mutant (lanes 4 and 5), using Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (top), LIMK1 Antibody #3842 (middle) or HA-Tag (262K) mAb #2362 (bottom). Cells were either untreated (lanes 1, 2 and 4) or treated with PMA (lanes 3 and 5). (Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.) 使用Phospho-LIMK1 (Thr508)/LIMK2 (Thr505) Antibody (上图)、LIMK1 Antibody #3842 (中图)或HA-Tag (262K) mAb #2362 (下图)，免疫印迹（Western Blot）分析COS细胞中未转染(第1道)、转染Wild-type LIMK1 (第2、3道)或转染LIMK1 T508A mutant (第4、5道)。细胞分为未处理组(第1、2、4道)和PMA处理(第3、5道)。(Triple HA-tagged LIMK1 plasmids kindly provided by Dr. K. Mizuno, Biological Institute, Tohoku University, Japan.) |
