| 货号 | 3921T |
| 反应种属 | Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody detects endogenous levels of GluR 2 only when phosphorylated at Tyr869, Tyr873 or Tyr876. It may also detect GluR 3 when phosphorylated at the conserved Tyr880, Tyr884 or Tyr887. These residues are not conserved in GluR 1 or GluR 4. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).Src family tyrosine kinases phosphorylate the GluR 2 subunit of AMPA receptors at Tyr876, which increases the interaction with GRIP1/2 but not PICK1. In addition, Tyr876 is important for AMPA- and NMDA-induced GluR 2 internalization (3).The phosphorylation sites at Tyr869, Tyr873 and Tyr876 were identified at Cell Signaling Technology (CST) using PhosphoScan®, CSTs MS/MS platform for phosphorylation site discovery. Phosphorylation of GluR2 at Tyr869, Tyr873 and Tyr876 was observed in extracts isolated from ischemic rat brain. These sites were independently found in a large-scale identification of tyrosine phosphorylation sites from murine brain (4). |
| 存放说明 | -20C |
| 计算分子量 | 100 |
| 参考文献 | 1 . Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77. 2 . Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97. 3 . Hayashi, T. and Huganir, R.L. (2004) J Neurosci 24, 6152-60. 4 . Ballif, B.A. et al. (2008) J Proteome Res 7, 311-8. |
Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody specificity was determined by peptide ELISA. The graphs depict the binding of the various dilutions of the antibody (no antibody, 1:1000, 1:750, 1:500, 1:250, 1:100) to various pre-coated GluR2 peptides. Phospho-AMPA Receptor (GluR 2) (Tyr869/Tyr873/Tyr876) Antibody 兔多抗的特异性由ELISA进行确认。图片显示了预包被GluR2的肽段与不同浓度（无抗体, 1:1000, 1:750, 1:500, 1:250, 1:100）的抗体结合。 | |
Western blot analysis of extracts from rat brain, either 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor (GluR 2) (Ty869/Tyr873/Tyr876) Antibody (upper) or AMPA Receptor (GluR 2/3/4) Antibody #2460 (lower). Western blot分析大鼠大脑（15分钟缺血后再灌注4小时或仅15分钟缺血），使用的抗体是Phospho-AMPA Receptor (GluR 2) (Ty869/Tyr873/Tyr876) Antibody 兔多抗（上图）或AMPA Receptor (GluR 2/3/4) Antibody 兔多抗#2460（下图）。 |
