| 货号 | 3983T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-ALK (Tyr1278/1282/1283) Antibody detects ALK only when phosphorylated at Tyr1278/1282/1283, which is equivalent to Tyr338/342/343 of NPM-ALK. This antibody might also have slight reactivity toward ALK when it is phosphorylated at Tyr1283 alone. This antibody also reacts with leukocyte tyrosine kinase (LTK) phosphorylated at Tyr672/676/677. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 80 (NPM-ALK), 220 (ALK) |
| 参考文献 | 1 . Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9. 2 . Iwahara, T. et al. (1997) Oncogene 14, 439-49. 3 . Morris, S.W. et al. (1997) Oncogene 14, 2175-88. 4 . Morris, S.W. et al. (1994) Science 263, 1281-4. 5 . Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61. 6 . Rikova, K. et al. (2007) Cell 131, 1190-203. 7 . Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24. 8 . Soda, M. et al. (2007) Nature 448, 561-6. |
Western blot analysis of extracts from KARPAS-299 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1278/1282/1283) Antibody (upper) and ALK (C26G7) Rabbit mAb #3333 (lower). |
