| 货号 | 4331T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-ATP-Citrate Lyase (Ser455) Antibody detects endogenous levels of ATP-citrate lyase only when phosphorylated at serine 455. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | ATP-citrate lyase (ACL) is a homotetramer that catalyzes the formation of acetyl-CoA and oxaloacetate (OAA) in the cytosol, which is the key step for the biosynthesis of fatty acids, cholestrol and acetylcholine, as well as for glucogenesis (1). Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2). It is phosphorylated by GSK-3 on Thr446 and Ser450 (3). Ser455 of ATP-citrate lyase has been reported to be phosphorylated by PKA and Akt (4,5). Phosphorylation on Ser455 abolishes the homotropic allosteric regulation by citrate and enhances the catalytic activity of the enzyme (2). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
| 参考文献 | 1 . Towle, H.C. et al. (1997) Annu Rev Nutr 17, 405-33. 2 . Potapova, I.A. et al. (2000) Biochemistry 39, 1169-79. 3 . Hughes, K. et al. (1992) Biochem J 288 ( Pt 1), 309-14. 4 . Pierce, M.W. et al. (1982) J Biol Chem 257, 10681-6. 5 . Berwick, D.C. et al. (2002) J Biol Chem 277, 33895-900. |
Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-treated for the indicated times, using Phospho-ATP-Citrate Lyase (Ser454) Antibody (upper) or ATP-Citrate Lyase Antibody #4332 (lower). Wetern blot检测NIH/3T3 提取物，未处理或用PDGF处理，所用抗体为Phospho-ATP-Citrate Lyase (Ser455) Antibody (上)和 ATP-Citrate Lyase Antibody #4332 (下)。 |
