| 货号 | 4441T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Stat2 Antibody detects endogenous levels of human Stat2 only when phosphorylated at Tyr690. The antibody does not significantly cross-react with corresponding phospho-tyrosine sites on other Stat proteins. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Stat2 (113-kDa), originally purified from the nuclei of alpha-interferon-treated cells, is critical to the transcriptional responses induced by type I interferons, IFN-alpha/beta (1,2). Knockout mice with a targeted disruption of Stat2 have higher susceptibility to viral infection and altered responses to type I interferons (3). Stat2 is rapidly activated by phosphorylation at Tyr690 in response to stimulation by IFN-alpha/beta via associations with receptor-bound Jak kinases (4). Unlike other Stat proteins, Stat2 does not form homodimers. Instead, activated Stat2 forms a heterodimer with Stat1 and translocates to the nucleus. There, it associates with the DNA-binding protein p48 and forms the transcriptional activator complex, interferon-stimulated gene factor 3 (ISGF3), promoting transcription from the ISRE (5). |
| 存放说明 | -20C |
| 计算分子量 | 113 |
| 参考文献 | 1 . Fu, X.Y. et al. (1992) Proc Natl Acad Sci U S A 89, 7840-3. 2 . Ihle, J.N. (2001) Curr Opin Cell Biol 13, 211-7. 3 . Park, C. et al. (2000) Immunity 13, 795-804. 4 . Improta, T. et al. (1994) Proc Natl Acad Sci U S A 91, 4776-80. 5 . Horvath, C.M. et al. (1996) Mol Cell Biol 16, 6957-64. |
Western blot analysis of extracts from HeLa cells, untreated or IFN-α-treated (100 ng/ml, 15 minutes), using Stat2 Antibody (upper) or Phospho-Stat2 (Tyr690) Antibody (lower).Western免疫印迹。用 Stat2 Antibody (上图) or Phospho-Stat2 (Tyr690) Antibody (下图)研究未经处理的和经IFN-alpha(100 ng/ml,15 min)处理的 HeLa 细胞的细胞提取液。 |
