Phospho-PBK/TOPK (Thr9) Antibody【科瑞奥博】

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Phospho-PBK/TOPK (Thr9) Antibody

货号： 4941T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	4941T
反应种属	Human,
来源宿主	Rabbit
应用	WB
目标/特异性	Phospho-PBK/TOPK (Thr9) Antibody detects endogenous levels of PBK/TOPK only when phosphorylated at threonine 9.
使用方法	WB(1:1000)

性能
供应商	CST
灵敏度	Endogenous
背景	PBK/TOPK is a serine/threonine kinase that is phosphorylated and active during mitosis (1). PBK/TOPK is composed of kinase subdomains and a carboxy-terminal PDZ-Binding domain, which is thought to interact with the tumor suppressor protein hDlg (1). Increased PBK/TOPK expression has been observed in highly proliferative malignant cell lines, and PBK/TOPK expression is strongly downregulated during terminal differentiation of HL-60 leukemic cells (2,3). PMA-induced kinase activity toward PBK/TOPK has been observed (4), and cdc2/cyclinB has been shown to phosphorylate PBK/TOPK in vitro, presumably at Thr9 (1). Potential substrates of PBK/TOPK include p38 MAPK and c-Myc (3,4).PBK/TOPK是一种丝氨酸/苏氨酸激酶，在有丝分裂过程中被磷酸化和活化(1)。PBK/TOPK由激酶亚结构域和一个羧基末端PDZ-结合结构域，该结构域被认为能够和肿瘤抑制蛋白hDlg相互作用(1)。在高度增殖的恶性肿瘤细胞系中已观察到PBK/TOPK的表达增加，而在终端分化的HL-60白血病细胞中观察到PBK/TOPK的显著下调(2,3)。PMA-诱导的针对PBK/TOPK的激酶活性也被观察到，且cdc2/cyclinB被认为可以离体磷酸化PBK/TOPK，可能的位点是第9位苏氨酸(1)。PBK/TOPK的潜在底物包括p38 MAPK 和c-Myc (3,4)。
存放说明	-20C
计算分子量	40
参考文献	1 . Gaudet, S. et al. (2000) Proc. Natl. Acad. Sci. U S A 97, 5167-5172. 2 . Simons-Evelyn, M. et al. (2001) Blood Cells Mol. Dis. 27, 825-829. 3 . Nandi, A. et al. (2004) Blood Cells Mol. Dis. 32, 240-245. 4 . Abe, Y. et al. (2000) J. Biol. Chem. 276, 21525-21531. 5 . Matsumoto, S. et al. (2004) Biochem Biophys Res Commun. 325, 997-1004.

参考图片

Confocal immunofluorescent analysis of HT-29 cells, untreated (left) or λ phosphatase-treated (right), using Phospho-PBK/TOPK (Thr9) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测HT-29细胞，分为非处理组（左图）和λ 磷酸酶处理组（右图），使用的抗体为Phospho-PBK/TOPK (Thr9) Antibody ，呈绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料，产品信息DRAQ5® #4084。

Western blot analysis of extracts from HT29 cells, untreated or nocodazole-treated (50ng/ml), using Phospho-PBK/TOPK (Thr9) Antibody (upper) or PBK/TOPK Antibody #4942 (lower).Western blot 方法检测HT29细胞提取物，分为未处理组和诺考达唑(50ng/ml)处理组,使用的抗体为 Phospho-PBK/TOPK (Thr9) Antibody (上图) 或 PBK/TOPK Antibody #4942 (下图)。

Flow cytometric analysis of untreated Jurkat cells, using Phospho-PBK/TOPK (Thr9) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-PBK/TOPK (Thr9)-positive cells.流式细胞术检测未处理的Jurkat细胞，使用的抗体为Phospho-PBK/TOPK (Thr9) Antibody ，碘化乙啶检测DNA含量作为对照。框内的细胞为磷酸化PBK/TOPK (Thr9)阳性的细胞。