| 货号 | 4547T |
| 反应种属 | Human,Mouse, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-PDGF Receptor α (Tyr1018) Antibody detects PDGFRα only when phosphorylated at Tyr1018. The antibody does not cross-react with activated PDGFRβ. But it may cross-react with other tyrosine phosphorylated protein tyrosine kinases including EGF receptor. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ, and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8). |
| 存放说明 | -20C |
| 计算分子量 | 190 |
| 参考文献 | 1 . Deuel, T.F. et al. (1988) Biofactors 1, 213-7. 2 . Bergsten, E. et al. (2001) Nat Cell Biol 3, 512-6. 3 . Betsholtz, C. et al. (2001) Bioessays 23, 494-507. 4 . Coughlin, S.R. et al. (1988) Prog Clin Biol Res 266, 39-45. 5 . Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38. 6 . Panayotou, G. et al. (1992) EMBO J 11, 4261-72. 7 . Ramalingam, K. et al. (1995) Bioorg Med Chem 3, 1263-72. 8 . Kashishian, A. et al. (1992) EMBO J 11, 1373-82. |
Western blot analysis of cell extracts from NIH/3T3 cells, untreated or treated with PDGF-AA, using Phospho-PDGF Receptor α (Tyr1018) Antibody (upper) or PDGF Receptor α Antibody #3164 (lower).用Phospho-PDGF Receptor α (Tyr1018) Antibody (上) 或PDGF Receptor α Antibody #3164 (下) 对未处理的或经过PDGF-AA处理的NIH/3T3细胞的提取物进行免疫印迹检测。 |
