| 货号 | 2181T |
| 反应种属 | Human,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB, IP |
| 目标/特异性 | Phospho-Rb (Ser608) Antibody detects endogenous levels of Rb only when phosphorylated at serine 608. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).视网膜母细胞瘤肿瘤抑制蛋白Rb通过控制细胞周期的G1期限制点的进程来调控细胞增殖(1)。Rb具有3个功能特异的结合结构域并能够与转录因子家族的关键调节蛋白E2F、c-Abl酪氨酸激酶和具有保守的LXCXE模体蛋白相互作用(2-4)。CDK介导的细胞周期依赖性磷酸化能够抑制Rb靶向结合并允许细胞周期进程(5)。Rb的失活和随后的细胞周期进程似乎需要伴随周期蛋白E-CDK2磷酸化的cyclin D-CDK4/6初始磷酸化(6)。离体已经观察到不同CDK/cyclin复合物的特异性(6-8)，而且周期蛋白D1为第780位丝氨酸在体磷酸化所必需(9)。 |
| 存放说明 | -20C |
| 计算分子量 | 110 |
| 参考文献 | 1 . Sherr, C.J. (1996) Science 274, 1672-7. 2 . Nevins, J.R. (1992) Science 258, 424-9. 3 . Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. 4 . Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. 5 . Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. 6 . Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. 7 . Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. 8 . Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. 9 . Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9. |
Western blot analysis of extracts from HeLa cells treated with lambda Phosphatase NEB #P0753 (10,000 units/ml, 1 h) or calyculin A #9902 (100 nM, 10 min) using Phospho-Rb (Ser608) Antibody (upper) or Rb (4H1) Mouse mAb #9309 (lower).Western blot方法检测Hela细胞提取物，分为lambda磷酸酶(10,000 units/ml, NEB #P0753)处理1小时组或calyculin A #9902 (100 nM)处理10分钟组，使用的抗体为Phospho-Rb (Ser608) Antibody (上图)和 Rb (4H1) Mouse mAb #9309 (下图)。 |
