| 货号 | 2083T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Raptor (Ser792) Antibody detects endogenous levels of raptor protein only when phosphorylated at Ser792. The antibody may also detect non-specific signals of various molecular weights. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets (1,2). Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates (3,4). Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycins specific inhibition of mTOR signaling (5). This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL (6). GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα (7). Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB (8). Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK) (9). AMPK phosphorylates raptor on Ser722/Ser792 (9). This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy (9). These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status. |
| 存放说明 | -20C |
| 计算分子量 | 150 |
| 参考文献 | 1 . Hara, K. et al. (2002) Cell 110, 177-89. 2 . Kim, D.H. et al. (2002) Cell 110, 163-75. 3 . Beugnet, A. et al. (2003) J Biol Chem 278, 40717-22. 4 . Nojima, H. et al. (2003) J Biol Chem 278, 15461-4. 5 . Oshiro, N. et al. (2004) Genes Cells 9, 359-366. 6 . Kim, D.H. et al. (2003) Mol. Cell 11, 895-904. 7 . Sarbassov, D.D. et al. (2004) Curr. Biol. 14, 1296-1302. 8 . Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 9 . Gwinn, D.M. et al. (2008) Mol Cell 30, 214-26. |
Western blot analysis of wild-type (WT) and AMPKα1 and α2 knockout (KO) mouse embryonic fibroblasts (MEFs), untreated or treated with AICAR (2 mM for 1 hour), using Phospho-Raptor (Ser792) Antibody (upper) or Raptor Antibody #4978 (lower). (Image provided by Dr. Reuben Shaw, Salk Institute for Biological Studies).*Cross-reacting bands at 60, 70 and 240 kDa. Western blot 方法检测野生型（WT）和 AMPKα1及α2 敲除（KO）的小鼠胚胎成纤维细胞(MEFs),使用的抗体是Phospho-Raptor (Ser792) Antibody （上图）和 Raptor Antibody #4978 （下图）。样品是未处理的或用AICAR (2 mM)处理一个小时的。（图片由Dr. Reuben Shaw, Salk Institute for Biological Studies提供）。交叉反应的条带大小为60, 70 和240 kDa。 |
