| 货号 | 5082T |
| 反应种属 | Human, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-Btk (Tyr223) Antibody detects endogenous levels of Btk only when phosphorylated at Tyr223. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Brutons tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity (8). |
| 存放说明 | -20C |
| 计算分子量 | 77 |
| 参考文献 | 1 . Khan, W.N. (2001) Immunol Res 23, 147-56. 2 . Lewis, C.M. et al. (2001) Curr Opin Immunol 13, 317-25. 3 . Salim, K. et al. (1996) EMBO J 15, 6241-50. 4 . Rameh, L.E. et al. (1997) J Biol Chem 272, 22059-66. 5 . Várnai, P. et al. (1999) J Biol Chem 274, 10983-9. 6 . Rawlings, D.J. et al. (1996) Science 271, 822-5. 7 . Park, H. et al. (1996) Immunity 4, 515-25. 8 . Kang, S.W. et al. (2001) EMBO J 20, 5692-702. |
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human IgM (12 µg/ml for 10 minutes), using Phospho-Btk (Tyr223) Antibody (upper) or Btk (C82B8) Rabbit mAb #3533 (lower).Western免疫印迹。用Phospho-Btk (Tyr223) Antibody (上图)或Btk (C82B8) Rabbit mAb #3533 (下图)研究未经处理的和经anti-human IgM (12 µg/ml ,10 min)处理的Ramos细胞的细胞提取液。 |
