| 货号 | 2054T |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-PKD/PKCμ (Ser744/748) Antibody detects PKD1/PKCμ only when dually phosphorylated at serines 744 and 748. This antibody may also cross-react with isoforms PKD2 and PKD3/PKCη in some species. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6). |
| 存放说明 | -20C |
| 计算分子量 | 115 |
| 参考文献 | 1 . Nishizuka, Y. (1984) Nature 308, 693-8. 2 . Keranen, L.M. et al. (1995) Curr Biol 5, 1394-403. 3 . Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576. 4 . Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148. 5 . Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667. 6 . Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549. 7 . Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51. 8 . Amadesi, S. et al. (2009) J Comp Neurol 516, 141-56. |
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser744/748) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower). |
