| 货号 | 2751T |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Lck (Tyr505) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr505. This antibody may cross-react with certain phosphorylated Src family members due to high sequence homology. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| 存放说明 | -20C |
| 计算分子量 | 56 |
| 参考文献 | 1 . Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609. 2 . Hunter, T. (1987) Cell 49, 1-4. 3 . Molina, T.J. et al. (1992) Nature 357, 161-4. 4 . Chow, L.M. et al. (1993) Nature 365, 156-60. 5 . Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93. |
Western blot analysis of extracts from Jurkat cells (starved for 16 hours) treated with calf intestinal alkaline phosphatase (CIP) or H2O2 (2 mM), using Phospho-Lck (Tyr505) Antibody (upper) or control Lck Antibody #2752 (lower).Western免疫印迹。用Phospho-Lck (Tyr505) Antibody (上图) 或 control Lck Antibody #2752 (下图) 研究经牛肠道碱性磷酸酶(CIP)处理或H2O2 (2 mM)处理的饥饿16小时Jurkat细胞的细胞提取液。 |
