| 货号 | 2731T |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | Phospho-Lyn (Tyr507) Antibody detects endogenous levels of Lyn only when phosphorylated at Tyr507. The antibody may cross-react with phospho-Lck (Tyr505) and phospho-Src (Tyr527) due to high sequence homology. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8). |
| 存放说明 | -20C |
| 计算分子量 | 53, 56 |
| 参考文献 | 1 . Yamanashi, Y. et al. (1989) Proc Natl Acad Sci U S A 86, 6538-42. 2 . Yamanashi, Y. et al. (1991) Science 251, 192-4. 3 . Burkhardt, A.L. et al. (1991) Proc Natl Acad Sci U S A 88, 7410-4. 4 . Ren, C.L. et al. (1994) J Exp Med 179, 673-80. 5 . Wang, J. et al. (1996) J Exp Med 184, 831-8. 6 . Chan, V.W. et al. (1997) Immunity 7, 69-81. 7 . Hibbs, M.L. et al. (1995) Cell 83, 301-11. 8 . Seo, S. et al. (2001) J. Immunol. 166, 3710-3716. |
Western blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12 µg/ml for 2 minutes), using Phospho-Lyn (Tyr507) Antibody. The phospho-specificity of the antibody was characterized by treating the membrane without or with calf intestinal alkaline phosphatase (CIP) after western transfer.Western免疫印迹。用Phospho-Lyn (Tyr507) Antibody研究经 anti-IgM antibody (12 µg/ml ,2 min)处理的Ramos细胞的细胞提取液。抗体对磷酸化的特异性是在转膜后通过用牛肠道碱性磷酸酶(CIP)或者不用此酶处理。 |
