| 货号 | 2715T |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Phospho-Syk (Tyr323) Antibody detects endogenous levels of Syk only when phosphorylated at Tyr323. It does not cross-react with other tyrosine-phosphorylated members of the Syk/Zap-70 tyrosine kinase family. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9). |
| 存放说明 | -20C |
| 计算分子量 | 72 |
| 参考文献 | 1 . Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33. 2 . Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18. 3 . Chu, D.H. et al. (1998) Immunol Rev 165, 167-80. 4 . Turner, M. et al. (2000) Immunol Today 21, 148-54. 5 . Coopman, P.J. et al. (2000) Nature 406, 742-7. 6 . Deckert, M. et al. (1998) J Biol Chem 273, 8867-74. 7 . Rao, N. et al. (2001) EMBO J 20, 7085-95. 8 . Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15. 9 . Zhang, J. et al. (2000) J Biol Chem 275, 35442-7. |
Western blot analysis of extracts from 293T cells expressing recombinant wild-type or mutant Syk proteins, cotransfected with CD8, using Phospho-Syk (Tyr323) Antibody (upper) or Syk Antibody #2712 (lower). (Provided by Dr. Alagarsamy L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)Western免疫印迹。用Phospho-Syk (Tyr323) Antibody #2715 (上图) 或者Syk Antibody #2712 (下图) (系Dr. Hamid Band实验室的Dr. Alagarsamy L. Reddi, 哈弗大学提供) 研究共转染CD8和野生型或者突变Syk蛋白的293T细胞的细胞提取液。 | |
Western blot analysis of extracts from Ramos cells, untreated or treated with anti-human-IgM (10 µg/ml), using Phospho-Syk (Tyr323) Antibody #2715 (upper) or Syk Antibody #2712 (lower).Western免疫印迹。用Phospho-Syk (Tyr323) Antibody #2715 (上图) 或者Syk Antibody #2712 (下图) 研究未经处理的和经anti-human-IgM (10 µg/ml) 处理的Ramos细胞的细胞提取液。 |
