| 货号 | 9208T |
| 反应种属 | Human,Mouse,Rat,Monkey, |
| 来源宿主 | Rabbit |
| 应用 | WB |
| 目标/特异性 | Phospho-p70 S6 Kinase (Ser371) Antibody detects endogenous levels of p70 S6 kinase only when phosphorylated at serine 371. This antibody also detects p85 S6 kinase when phosphorylated at the analogous site (Ser394) and possibly S6KII beta phosphorylated at Ser370. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (1,2). p70 S6 kinase phosphorylates the S6 protein of the 40S ribosomal subunit and is involved in translational control of 5 oligopyrimidine tract mRNAs (1). A second isoform, p85 S6 kinase, is derived from the same gene and is identical to p70 S6 kinase except for 23 extra residues at the amino terminus, which encode a nuclear localizing signal (1). Both isoforms lie on a mitogen activated signaling pathway downstream of phosphoinositide-3 kinase (PI-3K) and the target of rapamycin, FRAP/mTOR, a pathway distinct from the Ras/MAP kinase cascade (1). The activity of p70 S6 kinase is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains (1). Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function (1). Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo (3). Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229 (4,5). Phosphorylation of this site is stimulated by growth factors such as insulin, EGF and FGF, as well as by serum and some G-protein-coupled receptor ligands, and is blocked by wortmannin, LY294002 (PI-3K inhibitor) and rapamycin (FRAP/mTOR inhibitor) (1,6,7). Ser411, Thr421 and Ser424 lie within a Ser-Pro-rich region located in the pseudosubstrate region (1). Phosphorylation at these sites is thought to activate p70 S6 kinase via relief of pseudosubstrate suppression (1,2). Another LY294002 and rapamycin sensitive phosphorylation site, Ser371, is an in vitro substrate for mTOR and correlates well with the activity of a partially rapamycin resistant mutant p70 S6 kinase (8). |
| 存放说明 | -20C |
| 计算分子量 | 70, 85 |
| 参考文献 | 1 . Pullen, N. and Thomas, G. (1997) FEBS Lett 410, 78-82. 2 . Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9. 3 . Weng, Q.P. et al. (1998) J Biol Chem 273, 16621-9. 4 . Pullen, N. et al. (1998) Science 279, 707-10. 5 . Alessi, D.R. et al. (1998) Curr Biol 8, 69-81. 6 . Polakiewicz, R.D. et al. (1998) J Biol Chem 273, 23534-41. 7 . Fingar, D.C. et al. (2002) Genes Dev 16, 1472-87. 8 . Saitoh, M. et al. (2002) J Biol Chem 277, 20104-12. |
Western blot analysis of lysates from 293 cells grown in low serum, then treated with 20% serum for 30 minutes alone or after 1 hour preincubation with rapamycin (10nM) #9904 or LY294002 (50uM) #9901, using Phospho-p70 S6 Kinase (Ser371) Antibody (upper) or p70 S6 Kinase Antibody #9202 (lower). Western blot分析293细胞提取物，该细胞在低浓度血清中生长，然后采用20%血清处理30分钟或者与纳巴霉素(10nM) #9904或LY294002 (50uM) #9901孵育1小时，所用抗体为Phospho-p70 S6 Kinase (Ser371) Antibody (上) 或 p70 S6 Kinase Antibody #9202 (下)。 | |
Western blot analysis of lysates from unsynchronized (U) and nocodazole (N) treated (50ng/ml for 48 hours) HT29 cells using Phospho-p70 S6 Kinase (Ser371) Antibody (B) and p70 S6 Kinase Antibody #9202 (D). Incubation of the nitrocellulose membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer abolishes the phospho-p70 S6 Kinase signal (A), but has no effect on the total p70 S6 kinase signal (C). Western blot 分析非同步化（U）和噻氨酯哒唑（N）处理(50ng/ml for 48 hours)的HT29细胞，所用抗体为Phospho-p70 S6 Kinase (Ser371) Antibody (B) 和 p70 S6 Kinase Antibody #9202 (D)。western 转膜后，硝化纤维素膜与小牛肠碱性磷酸酶共孵育消除磷酸化p70S6激酶信号（A），但是对于总p70S6激酶信号无影响(C)。 |
