| 货号 | 9346T |
| 反应种属 | Human,Mouse,Rat, |
| 来源宿主 | Rabbit |
| 应用 | WB, IF-IC |
| 目标/特异性 | Phospho-p90RSK (Thr573) Antibody detects endogenous p90RSK protein only when phosphorylated at Thr573. |
| 使用方法 | WB(1:1000) IF-IC (1:400) |
| 供应商 | CST |
| 灵敏度 | Endogenous |
| 背景 | The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
| 参考文献 | 1 . Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9. 2 . Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8. 3 . Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. |
Western blot analysis of extracts from HeLa cells, untreated or TPA-treated (200 nM), using Phospho-p90RSK (Thr573) Antibody (upper) or RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (lower).Western blot方法检测细胞提取物：未经处理的和TPA(200 nM), 处理的HeLa细胞，使用的抗体是Phospho-p90RSK (Thr573) Antibody (上图) 和RSK1/RSK2/RSK3 (32D7) Rabbit mAb #9355 (下图). | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TPA-treated (right), using Phospho-p90RSK (Thr573) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，左侧是未经处理的样品，右侧是经TPA处理的样品，使用的抗体是Phospho-p90RSK (Thr573) Antibody（呈绿色）。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。 |
