Phospho-Stat6 (Tyr641) Antibody【科瑞奥博】

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Phospho-Stat6 (Tyr641) Antibody

货号： 9361T 基本售价： 1600.0 元规格： -

产品信息

概述
货号	9361T
反应种属	Human,
来源宿主	Rabbit
应用	WB, IP , F , IF-IC
目标/特异性	Phospho-Stat6 (Tyr641) Antibody detects endogenous levels of Stat6 only when phosphorylated at tyrosine 641. The antibody does not cross-react with the corresponding phospho-tyrosine residues of other Stat proteins.
使用方法	WB(1:1000) IP (1:100) F (1:100) IF-IC (1:100)

性能
供应商	CST
灵敏度	Endogenous
背景	Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cell lines, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).
存放说明	-20C
计算分子量	110
参考文献	1 . Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738. 2 . Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872. 3 . Hou, J. et al. (1994) Science 265, 1701-1706. 4 . Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343. 5 . Takeda, K. et al. (1996) Nature 380, 627-630. 6 . Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841. 7 . Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

参考图片

Confocal immunofluorescent analysis of ACHN cells serum-starved (left) or IL-4-treated (right) using Phospho-Stat6(Tyr641) Antibody (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).共聚焦免疫荧光。用Phospho-Stat6(Tyr641) 抗体 (绿色)分析血清饥饿(左图)或者IL-4处理(右图)。DRAQ5® #4084 (DNA荧光染料)(蓝色)。

Flow cytometric analysis of Ramos cells, untreated (blue) or IL-4 treated (green), using Phospho-Stat6 (Tyr641) Antibody compared to a nonspecific negative control antibody (red).流式细胞仪分析未经处理(蓝色)和经过 IL-4处理(绿色)的Ramos细胞。所用抗体为 Phospho-Stat6 (Tyr641) Antibody 与非特异性的阴性对照抗体(红色)。

Western blot analysis of extracts from Daudi cells, treated with IL-4 (100 ng/ml) for the indicated times using Phospho-Stat6 (Tyr641) Antibody (upper) or Stat6 antibody (lower).Western免疫印迹。用Phospho-Stat6 (Tyr641) 抗体 (上图) 或 Stat6 抗体 (下图)研究经过 IL-4 (100 ng/ml) 处理一定时间的Daudi细胞的细胞提取液。